Rescue of infectious Arumowot virus from cloned cDNA: Posttranslational degradation of Arumowot virus NSs protein in human cells
Identifieur interne : 000465 ( Main/Exploration ); précédent : 000464; suivant : 000466Rescue of infectious Arumowot virus from cloned cDNA: Posttranslational degradation of Arumowot virus NSs protein in human cells
Auteurs : Hoai J. Hallam [États-Unis] ; Nandadeva Lokugamage [États-Unis] ; Tetsuro Ikegami [États-Unis]Source :
- PLoS Neglected Tropical Diseases [ 1935-2727 ] ; 2019.
Abstract
Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and the Middle East, affecting both humans and ruminants. There are no licensed vaccines or antivirals available for humans, whereas research using RVF virus (RVFV) is strictly regulated in many countries with safety concerns. Nonpathogenic Arumowot virus (AMTV), a mosquito-borne phlebovirus in Africa, is likely useful for the screening of broad-acting antiviral candidates for phleboviruses including RVFV, as well as a potential vaccine vector for RVF. In this study, we aimed to generate T7 RNA polymerase-driven reverse genetics system for AMTV. We hypothesized that recombinant AMTV (rAMTV) is viable, and AMTV NSs protein is dispensable for efficient replication of rAMTV in type-I interferon (IFN)-incompetent cells, whereas AMTV NSs proteins support robust viral replication in type-I IFN-competent cells. The study demonstrated the rescue of rAMTV and that lacking the NSs gene (rAMTVΔNSs), that expressing green fluorescent protein (GFP) (rAMTV-GFP) or that expressing
Url:
DOI: 10.1371/journal.pntd.0007904
PubMed: 31751340
PubMed Central: 6894884
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000769
- to stream Pmc, to step Curation: 000769
- to stream Pmc, to step Checkpoint: 000421
- to stream Ncbi, to step Merge: 000C31
- to stream Ncbi, to step Curation: 000C31
- to stream Ncbi, to step Checkpoint: 000C31
- to stream Main, to step Merge: 000465
- to stream Main, to step Curation: 000465
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rescue of infectious Arumowot virus from cloned cDNA: Posttranslational degradation of Arumowot virus NSs protein in human cells</title><author><name sortKey="Hallam, Hoai J" sort="Hallam, Hoai J" uniqKey="Hallam H" first="Hoai J." last="Hallam">Hoai J. Hallam</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Lokugamage, Nandadeva" sort="Lokugamage, Nandadeva" uniqKey="Lokugamage N" first="Nandadeva" last="Lokugamage">Nandadeva Lokugamage</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Ikegami, Tetsuro" sort="Ikegami, Tetsuro" uniqKey="Ikegami T" first="Tetsuro" last="Ikegami">Tetsuro Ikegami</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff002"><addr-line>Sealy Institute for Vaccine Sciences, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Sealy Institute for Vaccine Sciences, The University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff003"><addr-line>Center for Biodefense and Emerging Infectious Diseases, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biodefense and Emerging Infectious Diseases, The University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31751340</idno><idno type="pmc">6894884</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894884</idno><idno type="RBID">PMC:6894884</idno><idno type="doi">10.1371/journal.pntd.0007904</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000769</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000769</idno><idno type="wicri:Area/Pmc/Curation">000769</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000769</idno><idno type="wicri:Area/Pmc/Checkpoint">000421</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000421</idno><idno type="wicri:Area/Ncbi/Merge">000C31</idno><idno type="wicri:Area/Ncbi/Curation">000C31</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C31</idno><idno type="wicri:doubleKey">1935-2727:2019:Hallam H:rescue:of:infectious</idno><idno type="wicri:Area/Main/Merge">000465</idno><idno type="wicri:Area/Main/Curation">000465</idno><idno type="wicri:Area/Main/Exploration">000465</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rescue of infectious Arumowot virus from cloned cDNA: Posttranslational degradation of Arumowot virus NSs protein in human cells</title><author><name sortKey="Hallam, Hoai J" sort="Hallam, Hoai J" uniqKey="Hallam H" first="Hoai J." last="Hallam">Hoai J. Hallam</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Lokugamage, Nandadeva" sort="Lokugamage, Nandadeva" uniqKey="Lokugamage N" first="Nandadeva" last="Lokugamage">Nandadeva Lokugamage</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Ikegami, Tetsuro" sort="Ikegami, Tetsuro" uniqKey="Ikegami T" first="Tetsuro" last="Ikegami">Tetsuro Ikegami</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff002"><addr-line>Sealy Institute for Vaccine Sciences, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Sealy Institute for Vaccine Sciences, The University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff003"><addr-line>Center for Biodefense and Emerging Infectious Diseases, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biodefense and Emerging Infectious Diseases, The University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author></analytic><series><title level="j">PLoS Neglected Tropical Diseases</title><idno type="ISSN">1935-2727</idno><idno type="eISSN">1935-2735</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and the Middle East, affecting both humans and ruminants. There are no licensed vaccines or antivirals available for humans, whereas research using RVF virus (RVFV) is strictly regulated in many countries with safety concerns. Nonpathogenic Arumowot virus (AMTV), a mosquito-borne phlebovirus in Africa, is likely useful for the screening of broad-acting antiviral candidates for phleboviruses including RVFV, as well as a potential vaccine vector for RVF. In this study, we aimed to generate T7 RNA polymerase-driven reverse genetics system for AMTV. We hypothesized that recombinant AMTV (rAMTV) is viable, and AMTV NSs protein is dispensable for efficient replication of rAMTV in type-I interferon (IFN)-incompetent cells, whereas AMTV NSs proteins support robust viral replication in type-I IFN-competent cells. The study demonstrated the rescue of rAMTV and that lacking the NSs gene (rAMTVΔNSs), that expressing green fluorescent protein (GFP) (rAMTV-GFP) or that expressing <italic>Renilla</italic> luciferase (rAMTV-rLuc) from cloned cDNA. The rAMTV-rLuc and the RVFV rMP12-rLuc showed a similar susceptibility to favipiravir or ribavirin. Interestingly, neither of rAMTV nor rAMTVΔNSs replicated efficiently in human MRC-5 or A549 cells, regardless of the presence of NSs gene. Little accumulation of AMTV NSs protein occurred in those cells, which was restored via treatment with proteasomal inhibitor MG132. In murine MEF or Hepa1-6 cells, rAMTV, but not rAMTVΔNSs, replicated efficiently, with an inhibition of IFN-β gene upregulation. This study showed an establishment of the first reverse genetics for AMTV, a lack of stability of AMTV NSs proteins in human cells, and an IFN-β gene antagonist function of AMTV NSs proteins in murine cells. The AMTV can be a nonpathogenic surrogate model for studying phleboviruses including RVFV.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Rottingen, Ja" uniqKey="Rottingen J">JA Rottingen</name></author><author><name sortKey="Gouglas, D" uniqKey="Gouglas D">D Gouglas</name></author><author><name sortKey="Feinberg, M" uniqKey="Feinberg M">M Feinberg</name></author><author><name sortKey="Plotkin, S" uniqKey="Plotkin S">S Plotkin</name></author><author><name sortKey="Raghavan, Kv" uniqKey="Raghavan K">KV Raghavan</name></author><author><name sortKey="Witty, A" uniqKey="Witty A">A Witty</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rolin, Ai" uniqKey="Rolin A">AI Rolin</name></author><author><name sortKey="Berrang Ford, L" uniqKey="Berrang Ford L">L Berrang-Ford</name></author><author><name sortKey="Kulkarni, Ma" uniqKey="Kulkarni M">MA Kulkarni</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pepin, M" uniqKey="Pepin M">M Pepin</name></author><author><name sortKey="Bouloy, M" uniqKey="Bouloy M">M Bouloy</name></author><author><name sortKey="Bird, Bh" uniqKey="Bird B">BH Bird</name></author><author><name sortKey="Kemp, A" uniqKey="Kemp A">A Kemp</name></author><author><name sortKey="Paweska, J" uniqKey="Paweska J">J Paweska</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Swanepoel, R" uniqKey="Swanepoel R">R Swanepoel</name></author><author><name sortKey="Coetzer, Jaw" uniqKey="Coetzer J">JAW Coetzer</name></author><author><name sortKey="Coetzer, Jaw" uniqKey="Coetzer J">JAW Coetzer</name></author><author><name sortKey="Tustin, Rc" uniqKey="Tustin R">RC Tustin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pekosz, A" uniqKey="Pekosz A">A Pekosz</name></author><author><name sortKey="He, B" uniqKey="He B">B He</name></author><author><name sortKey="Lamb, Ra" uniqKey="Lamb R">RA Lamb</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author><author><name sortKey="Won, S" uniqKey="Won S">S Won</name></author><author><name sortKey="Peters, Cj" uniqKey="Peters C">CJ Peters</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brennan, B" uniqKey="Brennan B">B Brennan</name></author><author><name sortKey="Li, P" uniqKey="Li P">P Li</name></author><author><name sortKey="Zhang, S" uniqKey="Zhang S">S Zhang</name></author><author><name sortKey="Li, A" uniqKey="Li A">A Li</name></author><author><name sortKey="Liang, M" uniqKey="Liang M">M Liang</name></author><author><name sortKey="Li, D" uniqKey="Li D">D Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rezelj, Vv" uniqKey="Rezelj V">VV Rezelj</name></author><author><name sortKey="Overby, Ak" uniqKey="Overby A">AK Overby</name></author><author><name sortKey="Elliott, Rm" uniqKey="Elliott R">RM Elliott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mendoza, Ca" uniqKey="Mendoza C">CA Mendoza</name></author><author><name sortKey="Ebihara, H" uniqKey="Ebihara H">H Ebihara</name></author><author><name sortKey="Yamaoka, S" uniqKey="Yamaoka S">S Yamaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Adams, Mj" uniqKey="Adams M">MJ Adams</name></author><author><name sortKey="Lefkowitz, Ej" uniqKey="Lefkowitz E">EJ Lefkowitz</name></author><author><name sortKey="King, Amq" uniqKey="King A">AMQ King</name></author><author><name sortKey="Harrach, B" uniqKey="Harrach B">B Harrach</name></author><author><name sortKey="Harrison, Rl" uniqKey="Harrison R">RL Harrison</name></author><author><name sortKey="Knowles, Nj" uniqKey="Knowles N">NJ Knowles</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Palacios, G" uniqKey="Palacios G">G Palacios</name></author><author><name sortKey="Da Rosa, At" uniqKey="Da Rosa A">AT da Rosa</name></author><author><name sortKey="Savji, N" uniqKey="Savji N">N Savji</name></author><author><name sortKey="Sze, W" uniqKey="Sze W">W Sze</name></author><author><name sortKey="Wick, I" uniqKey="Wick I">I Wick</name></author><author><name sortKey="Guzman, H" uniqKey="Guzman H">H Guzman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Palacios, G" uniqKey="Palacios G">G Palacios</name></author><author><name sortKey="Tesh, R" uniqKey="Tesh R">R Tesh</name></author><author><name sortKey="Travassos Da Rosa, A" uniqKey="Travassos Da Rosa A">A Travassos da Rosa</name></author><author><name sortKey="Savji, N" uniqKey="Savji N">N Savji</name></author><author><name sortKey="Sze, W" uniqKey="Sze W">W Sze</name></author><author><name sortKey="Jain, K" uniqKey="Jain K">K Jain</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Palacios, G" uniqKey="Palacios G">G Palacios</name></author><author><name sortKey="Savji, N" uniqKey="Savji N">N Savji</name></author><author><name sortKey="Travassos Da Rosa, A" uniqKey="Travassos Da Rosa A">A Travassos da Rosa</name></author><author><name sortKey="Desai, A" uniqKey="Desai A">A Desai</name></author><author><name sortKey="Sanchez Seco, Mp" uniqKey="Sanchez Seco M">MP Sanchez-Seco</name></author><author><name sortKey="Guzman, H" uniqKey="Guzman H">H Guzman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Palacios, G" uniqKey="Palacios G">G Palacios</name></author><author><name sortKey="Tesh, Rb" uniqKey="Tesh R">RB Tesh</name></author><author><name sortKey="Savji, N" uniqKey="Savji N">N Savji</name></author><author><name sortKey="Travassos Da Rosa, Ap" uniqKey="Travassos Da Rosa A">AP Travassos da Rosa</name></author><author><name sortKey="Guzman, H" uniqKey="Guzman H">H Guzman</name></author><author><name sortKey="Bussetti, Av" uniqKey="Bussetti A">AV Bussetti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Palacios, G" uniqKey="Palacios G">G Palacios</name></author><author><name sortKey="Wiley, Mr" uniqKey="Wiley M">MR Wiley</name></author><author><name sortKey="Travassos Da Rosa, Ap" uniqKey="Travassos Da Rosa A">AP Travassos da Rosa</name></author><author><name sortKey="Guzman, H" uniqKey="Guzman H">H Guzman</name></author><author><name sortKey="Quiroz, E" uniqKey="Quiroz E">E Quiroz</name></author><author><name sortKey="Savji, N" uniqKey="Savji N">N Savji</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tesh, Rb" uniqKey="Tesh R">RB Tesh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kemp, Ge" uniqKey="Kemp G">GE Kemp</name></author><author><name sortKey="Causey, Or" uniqKey="Causey O">OR Causey</name></author><author><name sortKey="Setzer, Hw" uniqKey="Setzer H">HW Setzer</name></author><author><name sortKey="Moore, Dl" uniqKey="Moore D">DL Moore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Berthet, N" uniqKey="Berthet N">N Berthet</name></author><author><name sortKey="Nakoune, E" uniqKey="Nakoune E">E Nakoune</name></author><author><name sortKey="Gessain, A" uniqKey="Gessain A">A Gessain</name></author><author><name sortKey="Manuguerra, Jc" uniqKey="Manuguerra J">JC Manuguerra</name></author><author><name sortKey="Kazanji, M" uniqKey="Kazanji M">M Kazanji</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gonzalez, Jp" uniqKey="Gonzalez J">JP Gonzalez</name></author><author><name sortKey="Le Guenno, B" uniqKey="Le Guenno B">B Le Guenno</name></author><author><name sortKey="Some, Mj" uniqKey="Some M">MJ Some</name></author><author><name sortKey="Akakpo, Ja" uniqKey="Akakpo J">JA Akakpo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tesh, Rb" uniqKey="Tesh R">RB Tesh</name></author><author><name sortKey="Saidi, S" uniqKey="Saidi S">S Saidi</name></author><author><name sortKey="Gajdamovic, Sj" uniqKey="Gajdamovic S">SJ Gajdamovic</name></author><author><name sortKey="Rodhain, F" uniqKey="Rodhain F">F Rodhain</name></author><author><name sortKey="Vesenjak Hirjan, J" uniqKey="Vesenjak Hirjan J">J Vesenjak-Hirjan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Swanepoel, R" uniqKey="Swanepoel R">R Swanepoel</name></author><author><name sortKey="Struthers, Jk" uniqKey="Struthers J">JK Struthers</name></author><author><name sortKey="Erasmus, Mj" uniqKey="Erasmus M">MJ Erasmus</name></author><author><name sortKey="Shepherd, Sp" uniqKey="Shepherd S">SP Shepherd</name></author><author><name sortKey="Mcgillivray, Gm" uniqKey="Mcgillivray G">GM McGillivray</name></author><author><name sortKey="Shepherd, Aj" uniqKey="Shepherd A">AJ Shepherd</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tesh, Rb" uniqKey="Tesh R">RB Tesh</name></author><author><name sortKey="Duboise, Sm" uniqKey="Duboise S">SM Duboise</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kalveram, B" uniqKey="Kalveram B">B Kalveram</name></author><author><name sortKey="Lihoradova, O" uniqKey="Lihoradova O">O Lihoradova</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K Narayanan</name></author><author><name sortKey="Won, S" uniqKey="Won S">S Won</name></author><author><name sortKey="Kamitani, W" uniqKey="Kamitani W">W Kamitani</name></author><author><name sortKey="Peters, Cj" uniqKey="Peters C">CJ Peters</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Habjan, M" uniqKey="Habjan M">M Habjan</name></author><author><name sortKey="Pichlmair, A" uniqKey="Pichlmair A">A Pichlmair</name></author><author><name sortKey="Elliott, Rm" uniqKey="Elliott R">RM Elliott</name></author><author><name sortKey="Overby, Ak" uniqKey="Overby A">AK Overby</name></author><author><name sortKey="Glatter, T" uniqKey="Glatter T">T Glatter</name></author><author><name sortKey="Gstaiger, M" uniqKey="Gstaiger M">M Gstaiger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kainulainen, M" uniqKey="Kainulainen M">M Kainulainen</name></author><author><name sortKey="Habjan, M" uniqKey="Habjan M">M Habjan</name></author><author><name sortKey="Hubel, P" uniqKey="Hubel P">P Hubel</name></author><author><name sortKey="Busch, L" uniqKey="Busch L">L Busch</name></author><author><name sortKey="Lau, S" uniqKey="Lau S">S Lau</name></author><author><name sortKey="Colinge, J" uniqKey="Colinge J">J Colinge</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kainulainen, M" uniqKey="Kainulainen M">M Kainulainen</name></author><author><name sortKey="Lau, S" uniqKey="Lau S">S Lau</name></author><author><name sortKey="Samuel, Ce" uniqKey="Samuel C">CE Samuel</name></author><author><name sortKey="Hornung, V" uniqKey="Hornung V">V Hornung</name></author><author><name sortKey="Weber, F" uniqKey="Weber F">F Weber</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mudhasani, R" uniqKey="Mudhasani R">R Mudhasani</name></author><author><name sortKey="Tran, Jp" uniqKey="Tran J">JP Tran</name></author><author><name sortKey="Retterer, C" uniqKey="Retterer C">C Retterer</name></author><author><name sortKey="Kota, Kp" uniqKey="Kota K">KP Kota</name></author><author><name sortKey="Whitehouse, Ca" uniqKey="Whitehouse C">CA Whitehouse</name></author><author><name sortKey="Bavari, S" uniqKey="Bavari S">S Bavari</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Le May, N" uniqKey="Le May N">N Le May</name></author><author><name sortKey="Mansuroglu, Z" uniqKey="Mansuroglu Z">Z Mansuroglu</name></author><author><name sortKey="Leger, P" uniqKey="Leger P">P Leger</name></author><author><name sortKey="Josse, T" uniqKey="Josse T">T Josse</name></author><author><name sortKey="Blot, G" uniqKey="Blot G">G Blot</name></author><author><name sortKey="Billecocq, A" uniqKey="Billecocq A">A Billecocq</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Le May, N" uniqKey="Le May N">N Le May</name></author><author><name sortKey="Dubaele, S" uniqKey="Dubaele S">S Dubaele</name></author><author><name sortKey="Proietti De Santis, L" uniqKey="Proietti De Santis L">L Proietti De Santis</name></author><author><name sortKey="Billecocq, A" uniqKey="Billecocq A">A Billecocq</name></author><author><name sortKey="Bouloy, M" uniqKey="Bouloy M">M Bouloy</name></author><author><name sortKey="Egly, Jm" uniqKey="Egly J">JM Egly</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Billecocq, A" uniqKey="Billecocq A">A Billecocq</name></author><author><name sortKey="Spiegel, M" uniqKey="Spiegel M">M Spiegel</name></author><author><name sortKey="Vialat, P" uniqKey="Vialat P">P Vialat</name></author><author><name sortKey="Kohl, A" uniqKey="Kohl A">A Kohl</name></author><author><name sortKey="Weber, F" uniqKey="Weber F">F Weber</name></author><author><name sortKey="Bouloy, M" uniqKey="Bouloy M">M Bouloy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, X" uniqKey="Wu X">X Wu</name></author><author><name sortKey="Qi, X" uniqKey="Qi X">X Qi</name></author><author><name sortKey="Qu, B" uniqKey="Qu B">B Qu</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author><author><name sortKey="Liang, M" uniqKey="Liang M">M Liang</name></author><author><name sortKey="Li, C" uniqKey="Li C">C Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Santiago, Fw" uniqKey="Santiago F">FW Santiago</name></author><author><name sortKey="Covaleda, Lm" uniqKey="Covaleda L">LM Covaleda</name></author><author><name sortKey="Sanchez Aparicio, Mt" uniqKey="Sanchez Aparicio M">MT Sanchez-Aparicio</name></author><author><name sortKey="Silvas, Ja" uniqKey="Silvas J">JA Silvas</name></author><author><name sortKey="Diaz Vizarreta, Ac" uniqKey="Diaz Vizarreta A">AC Diaz-Vizarreta</name></author><author><name sortKey="Patel, Jr" uniqKey="Patel J">JR Patel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ning, Yj" uniqKey="Ning Y">YJ Ning</name></author><author><name sortKey="Feng, K" uniqKey="Feng K">K Feng</name></author><author><name sortKey="Min, Yq" uniqKey="Min Y">YQ Min</name></author><author><name sortKey="Cao, Wc" uniqKey="Cao W">WC Cao</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Deng, F" uniqKey="Deng F">F Deng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ito, N" uniqKey="Ito N">N Ito</name></author><author><name sortKey="Takayama Ito, M" uniqKey="Takayama Ito M">M Takayama-Ito</name></author><author><name sortKey="Yamada, K" uniqKey="Yamada K">K Yamada</name></author><author><name sortKey="Hosokawa, J" uniqKey="Hosokawa J">J Hosokawa</name></author><author><name sortKey="Sugiyama, M" uniqKey="Sugiyama M">M Sugiyama</name></author><author><name sortKey="Minamoto, N" uniqKey="Minamoto N">N Minamoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author><author><name sortKey="Peters, Cj" uniqKey="Peters C">CJ Peters</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kalveram, B" uniqKey="Kalveram B">B Kalveram</name></author><author><name sortKey="Lihoradova, O" uniqKey="Lihoradova O">O Lihoradova</name></author><author><name sortKey="Indran, Sv" uniqKey="Indran S">SV Indran</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author><author><name sortKey="Won, S" uniqKey="Won S">S Won</name></author><author><name sortKey="Peters, Cj" uniqKey="Peters C">CJ Peters</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ly, Hj" uniqKey="Ly H">HJ Ly</name></author><author><name sortKey="Lokugamage, N" uniqKey="Lokugamage N">N Lokugamage</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Diaz, Mo" uniqKey="Diaz M">MO Diaz</name></author><author><name sortKey="Ziemin, S" uniqKey="Ziemin S">S Ziemin</name></author><author><name sortKey="Le Beau, Mm" uniqKey="Le Beau M">MM Le Beau</name></author><author><name sortKey="Pitha, P" uniqKey="Pitha P">P Pitha</name></author><author><name sortKey="Smith, Sd" uniqKey="Smith S">SD Smith</name></author><author><name sortKey="Chilcote, Rr" uniqKey="Chilcote R">RR Chilcote</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mosca, Jd" uniqKey="Mosca J">JD Mosca</name></author><author><name sortKey="Pitha, Pm" uniqKey="Pitha P">PM Pitha</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brennan, B" uniqKey="Brennan B">B Brennan</name></author><author><name sortKey="Welch, Sr" uniqKey="Welch S">SR Welch</name></author><author><name sortKey="Elliott, Rm" uniqKey="Elliott R">RM Elliott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lokugamage, N" uniqKey="Lokugamage N">N Lokugamage</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kalveram, B" uniqKey="Kalveram B">B Kalveram</name></author><author><name sortKey="Lihoradova, O" uniqKey="Lihoradova O">O Lihoradova</name></author><author><name sortKey="Indran, Sv" uniqKey="Indran S">SV Indran</name></author><author><name sortKey="Lokugamage, N" uniqKey="Lokugamage N">N Lokugamage</name></author><author><name sortKey="Head, Ja" uniqKey="Head J">JA Head</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author><author><name sortKey="Hill, Te" uniqKey="Hill T">TE Hill</name></author><author><name sortKey="Smith, Jk" uniqKey="Smith J">JK Smith</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L Zhang</name></author><author><name sortKey="Juelich, Tl" uniqKey="Juelich T">TL Juelich</name></author><author><name sortKey="Gong, B" uniqKey="Gong B">B Gong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Verhaegen, M" uniqKey="Verhaegen M">M Verhaegen</name></author><author><name sortKey="Divizia, M" uniqKey="Divizia M">M Divizia</name></author><author><name sortKey="Vandenbussche, P" uniqKey="Vandenbussche P">P Vandenbussche</name></author><author><name sortKey="Kuwata, T" uniqKey="Kuwata T">T Kuwata</name></author><author><name sortKey="Content, J" uniqKey="Content J">J Content</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Constantinescu, Sn" uniqKey="Constantinescu S">SN Constantinescu</name></author><author><name sortKey="Croze, E" uniqKey="Croze E">E Croze</name></author><author><name sortKey="Murti, A" uniqKey="Murti A">A Murti</name></author><author><name sortKey="Wang, C" uniqKey="Wang C">C Wang</name></author><author><name sortKey="Basu, L" uniqKey="Basu L">L Basu</name></author><author><name sortKey="Hollander, D" uniqKey="Hollander D">D Hollander</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lang, Y" uniqKey="Lang Y">Y Lang</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Jasperson, D" uniqKey="Jasperson D">D Jasperson</name></author><author><name sortKey="Henningson, J" uniqKey="Henningson J">J Henningson</name></author><author><name sortKey="Lee, J" uniqKey="Lee J">J Lee</name></author><author><name sortKey="Ma, J" uniqKey="Ma J">J Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Benedict, A" uniqKey="Benedict A">A Benedict</name></author><author><name sortKey="Bansal, N" uniqKey="Bansal N">N Bansal</name></author><author><name sortKey="Senina, S" uniqKey="Senina S">S Senina</name></author><author><name sortKey="Hooper, I" uniqKey="Hooper I">I Hooper</name></author><author><name sortKey="Lundberg, L" uniqKey="Lundberg L">L Lundberg</name></author><author><name sortKey="De La Fuente, C" uniqKey="De La Fuente C">C de la Fuente</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nuss, Je" uniqKey="Nuss J">JE Nuss</name></author><author><name sortKey="Kehn Hall, K" uniqKey="Kehn Hall K">K Kehn-Hall</name></author><author><name sortKey="Benedict, A" uniqKey="Benedict A">A Benedict</name></author><author><name sortKey="Costantino, J" uniqKey="Costantino J">J Costantino</name></author><author><name sortKey="Ward, M" uniqKey="Ward M">M Ward</name></author><author><name sortKey="Peyser, Bd" uniqKey="Peyser B">BD Peyser</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Narayanan, A" uniqKey="Narayanan A">A Narayanan</name></author><author><name sortKey="Kehn Hall, K" uniqKey="Kehn Hall K">K Kehn-Hall</name></author><author><name sortKey="Senina, S" uniqKey="Senina S">S Senina</name></author><author><name sortKey="Lundberg, L" uniqKey="Lundberg L">L Lundberg</name></author><author><name sortKey="Van Duyne, R" uniqKey="Van Duyne R">R Van Duyne</name></author><author><name sortKey="Guendel, I" uniqKey="Guendel I">I Guendel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gowen, Bb" uniqKey="Gowen B">BB Gowen</name></author><author><name sortKey="Wong, Mh" uniqKey="Wong M">MH Wong</name></author><author><name sortKey="Jung, Kh" uniqKey="Jung K">KH Jung</name></author><author><name sortKey="Sanders, Ab" uniqKey="Sanders A">AB Sanders</name></author><author><name sortKey="Mendenhall, M" uniqKey="Mendenhall M">M Mendenhall</name></author><author><name sortKey="Bailey, Kw" uniqKey="Bailey K">KW Bailey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mori, M" uniqKey="Mori M">M Mori</name></author><author><name sortKey="Yoneyama, M" uniqKey="Yoneyama M">M Yoneyama</name></author><author><name sortKey="Ito, T" uniqKey="Ito T">T Ito</name></author><author><name sortKey="Takahashi, K" uniqKey="Takahashi K">K Takahashi</name></author><author><name sortKey="Inagaki, F" uniqKey="Inagaki F">F Inagaki</name></author><author><name sortKey="Fujita, T" uniqKey="Fujita T">T Fujita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Panne, D" uniqKey="Panne D">D Panne</name></author><author><name sortKey="Mcwhirter, Sm" uniqKey="Mcwhirter S">SM McWhirter</name></author><author><name sortKey="Maniatis, T" uniqKey="Maniatis T">T Maniatis</name></author><author><name sortKey="Harrison, Sc" uniqKey="Harrison S">SC Harrison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Clement, Jf" uniqKey="Clement J">JF Clement</name></author><author><name sortKey="Bibeau Poirier, A" uniqKey="Bibeau Poirier A">A Bibeau-Poirier</name></author><author><name sortKey="Gravel, Sp" uniqKey="Gravel S">SP Gravel</name></author><author><name sortKey="Grandvaux, N" uniqKey="Grandvaux N">N Grandvaux</name></author><author><name sortKey="Bonneil, E" uniqKey="Bonneil E">E Bonneil</name></author><author><name sortKey="Thibault, P" uniqKey="Thibault P">P Thibault</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Verani, P" uniqKey="Verani P">P Verani</name></author><author><name sortKey="Ciufolini, Mg" uniqKey="Ciufolini M">MG Ciufolini</name></author><author><name sortKey="Caciolli, S" uniqKey="Caciolli S">S Caciolli</name></author><author><name sortKey="Renzi, A" uniqKey="Renzi A">A Renzi</name></author><author><name sortKey="Nicoletti, L" uniqKey="Nicoletti L">L Nicoletti</name></author><author><name sortKey="Sabatinelli, G" uniqKey="Sabatinelli G">G Sabatinelli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bichaud, L" uniqKey="Bichaud L">L Bichaud</name></author><author><name sortKey="Dachraoui, K" uniqKey="Dachraoui K">K Dachraoui</name></author><author><name sortKey="Alwassouf, S" uniqKey="Alwassouf S">S Alwassouf</name></author><author><name sortKey="Alkan, C" uniqKey="Alkan C">C Alkan</name></author><author><name sortKey="Mensi, M" uniqKey="Mensi M">M Mensi</name></author><author><name sortKey="Piorkowski, G" uniqKey="Piorkowski G">G Piorkowski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Papa, A" uniqKey="Papa A">A Papa</name></author><author><name sortKey="Velo, E" uniqKey="Velo E">E Velo</name></author><author><name sortKey="Bino, S" uniqKey="Bino S">S Bino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alkan, C" uniqKey="Alkan C">C Alkan</name></author><author><name sortKey="Alwassouf, S" uniqKey="Alwassouf S">S Alwassouf</name></author><author><name sortKey="Piorkowski, G" uniqKey="Piorkowski G">G Piorkowski</name></author><author><name sortKey="Bichaud, L" uniqKey="Bichaud L">L Bichaud</name></author><author><name sortKey="Tezcan, S" uniqKey="Tezcan S">S Tezcan</name></author><author><name sortKey="Dincer, E" uniqKey="Dincer E">E Dincer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Amaro, F" uniqKey="Amaro F">F Amaro</name></author><author><name sortKey="Ze Ze, L" uniqKey="Ze Ze L">L Ze-Ze</name></author><author><name sortKey="Alves, Mj" uniqKey="Alves M">MJ Alves</name></author><author><name sortKey="Borstler, J" uniqKey="Borstler J">J Borstler</name></author><author><name sortKey="Clos, J" uniqKey="Clos J">J Clos</name></author><author><name sortKey="Lorenzen, S" uniqKey="Lorenzen S">S Lorenzen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Calzolari, M" uniqKey="Calzolari M">M Calzolari</name></author><author><name sortKey="Chiapponi, C" uniqKey="Chiapponi C">C Chiapponi</name></author><author><name sortKey="Bellini, R" uniqKey="Bellini R">R Bellini</name></author><author><name sortKey="Bonilauri, P" uniqKey="Bonilauri P">P Bonilauri</name></author><author><name sortKey="Lelli, D" uniqKey="Lelli D">D Lelli</name></author><author><name sortKey="Moreno, A" uniqKey="Moreno A">A Moreno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peyrefitte, Cn" uniqKey="Peyrefitte C">CN Peyrefitte</name></author><author><name sortKey="Grandadam, M" uniqKey="Grandadam M">M Grandadam</name></author><author><name sortKey="Bessaud, M" uniqKey="Bessaud M">M Bessaud</name></author><author><name sortKey="Andry, Pe" uniqKey="Andry P">PE Andry</name></author><author><name sortKey="Fouque, F" uniqKey="Fouque F">F Fouque</name></author><author><name sortKey="Caro, V" uniqKey="Caro V">V Caro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ly, Hj" uniqKey="Ly H">HJ Ly</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kalveram, B" uniqKey="Kalveram B">B Kalveram</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gori Savellini, G" uniqKey="Gori Savellini G">G Gori-Savellini</name></author><author><name sortKey="Valentini, M" uniqKey="Valentini M">M Valentini</name></author><author><name sortKey="Cusi, Mg" uniqKey="Cusi M">MG Cusi</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Hallam, Hoai J" sort="Hallam, Hoai J" uniqKey="Hallam H" first="Hoai J." last="Hallam">Hoai J. Hallam</name></region><name sortKey="Ikegami, Tetsuro" sort="Ikegami, Tetsuro" uniqKey="Ikegami T" first="Tetsuro" last="Ikegami">Tetsuro Ikegami</name><name sortKey="Ikegami, Tetsuro" sort="Ikegami, Tetsuro" uniqKey="Ikegami T" first="Tetsuro" last="Ikegami">Tetsuro Ikegami</name><name sortKey="Ikegami, Tetsuro" sort="Ikegami, Tetsuro" uniqKey="Ikegami T" first="Tetsuro" last="Ikegami">Tetsuro Ikegami</name><name sortKey="Lokugamage, Nandadeva" sort="Lokugamage, Nandadeva" uniqKey="Lokugamage N" first="Nandadeva" last="Lokugamage">Nandadeva Lokugamage</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000465 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000465 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:6894884
|texte= Rescue of infectious Arumowot virus from cloned cDNA: Posttranslational degradation of Arumowot virus NSs protein in human cells
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31751340" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ChloroquineV1
| This area was generated with Dilib version V0.6.33. |  |